บทคัดย่องานวิจัย

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First report of thiabendazole-resistant isolates of Fusarium sambucinum infecting stored potatoes in Nova Scotia, Canada.

Peters, R. D., Macdonald, I. K., MacIsaac, K. A. and Woodworth, S.

Plant Disease, Disease Notes. Volume 85, Number 9, September 2001. Page 1030.

2001

บทคัดย่อ

First report of thiabendazole-resistant isolates of Fusarium sambucinum infecting stored potatoes in Nova Scotia, Canada.

Samples of potato tubers showing symptoms of dry rot caused by Fusarium spp. were collected from three storage bays in a commercial storage facility in Nova Scotia, Canada, in February 2001. All tubers had been treated with thiabendazole after harvest and prior to storage. Small tissue samples were taken from the margins of internal necrotic regions of tubers and plated on potato dextrose agar (PDA) with 0.05 g tetracycline/litre and 0.1 g streptomycin sulfate/litre. Of 35 potato tubers examined, 10 (29%) yielded Fusarium isolates. All 10 isolates were identified as F. sambucinum [Gibberella pulicaris]. Agar plugs (5 mm diameter) taken from the margins of 7- to 10-day-old cultures of F. sambucinum isolates were transferred to Petri dishes containing PDA amended with 0, 1, 5, 10, 20, 50 or 100 mg thiabendazole/litre. All isolates of F. sambucinum were resistant to thiabendazole, with EC50 values ranging from 7 to 82 mg/litre. Six isolates had EC50 values between 40 and 82 mg/litre. Control isolates of F. sambucinum, F. avenaceum, F. solani and F. oxysporum were sensitive to thiabendazole, with EC50 values of <1 mg/litre. Although isolates of F. sambucinum resistant to thiabendazole have been recovered from eastern Canada, this is thought to be the first report of thiabendazole resistance in F. sambucinum isolates from tubers in commercial storage in Annapolis Valley of Nova Scotia, Canada.