ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Isolation of two anthocyanin sambubiosides from bilberry (Vaccinium myrtillus) by high-speed counter-current chromatography

Qizhen Du , Gerold Jerz and Peter Winterhalter

Journal of Chromatography A Volume 1045, Issues 1-2 , 6 August 2004, Pages 59-63

2004

บทคัดย่อ

High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanins o­n a preparative scale from bilberry fruitcrude extract (Vaccinium myrtillus, Ericaceae) with a biphasic solvent system composed of methyl tert-butyl ether–n-butanol–acetonitrile–water–trifluoroacetic acid (1:4:1:5:0.01, v/v). Each injection of 500 mg crude extract yielded 130 mg of pure delphinidin-3-O-sambubioside, and 77 mg of pure cyanidin-3-O-sambubioside. The two anthocyanin disaccharides with a glucose (2 ®1) xylose unit are novel compounds for bilberryfruit,and were elucidated by means of electrospray ionization MS–MS, ¹H, ¹³C, distortion less enhancement by polarization transferNMR,and two-dimensional HC-correlation experiments (heteronuclear multiple bond correlation and heteronuclear multiple quantum correlation).