Crystallization and preliminary X-ray crystallographic analysis of plant ferritin from Glycine max
Taro Masuda, Bunzo Mikami, Fumiyuki Goto, Toshihiro Yoshihara and Shigeru Utsumi
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Volume 1645, Issue 1 , 31 January 2003, Pages 113-115
2003
บทคัดย่อ
Crystallization and preliminary X-ray crystallographic analysis of plant ferritin from Glycine max
The iron
storage protein ferritin from
soybean (
Glycine max) was expressed in
E. coli and crystallized using the hanging drop vapor diffusion method with sodium tartrate as the precipitant. The crystals belong to the tetragonal
I4
122 space group, with unit cell parameters
a=
b=324.0,
c=182.7 Å. The diffraction data were collected up to a resolution of 3.0 Å with a multi-wire area detector