Genomic approaches to understanding ripening control and fruit quality in tomato
Giovannoni, J., Alba, R., Vrebalov, J., Fei, Z., Liu, Y.
5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004. page 4
2004
บทคัดย่อ
Genomic approaches to understanding ripening control and fruit quality in tomato
As part of the National Science Foundation (NSF) supported Tomato Genome Project our laboratory has and continues to participate in the development of tomato genomics resources.Project participants (S. Tanksley, G. Martin, R. Wing) in collaboration with The Institute for Genome Research (TIGRI) have created over 150,000 tomato EST sequences from 23 different tissue samples.We are exploiting this EST collection develop microarrays which we employ for gene expression profiling to gain more depth in our analysis of fruit development and ripening.Ten stages from 6 days post-anthesis through late ripening have selected for expression analysis and the expression profile of normal fruit development has been competes.A current focus is on novel transcription factors associated with ripening and discovered through this analysis.A user-friendly Tomato Expression Database (TED) has also been developed as a means to transfer the resulting expression data to the research community (http://ted.bti.comell.edu/).The laboratory is also directing considerable effort toward characterization of developmental horonal and environmental (esp. light) signal transduction systems impacting maturation and quality characteristics associated with ripening through characterization and analysis of available tomato mutants.Positional cloning efforts resulting in isolation of the RIPENING-INHIBITOR (NOR) and NON-RIPENING (RIN)genes involved in developmental regulation of ripening indicate the NOR product is required for RIN gene expression.Progress toward positional cloning of additional tomato fruit mutants impacting quality (high-pigment-1) and abscission (jontless2) will be summarized.