The role of methyl jasmonate in ethylene biosynthsis and fruit ripening in strawberries
Mukkun, L., Zora Singh
5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004. page 4
2004
บทคัดย่อ
The role of methyl jasmonate in ethylene biosynthsis and fruit ripening in strawberries
The role of methyl jasmonate (MJ) in
‘Pafaro
’ strawberry fruit ripening was investigated by nomitoring endogenous MJ levels at various stages of fruit ripening including white, half ripe and at fully ripe and with its exogenous application.Endogenous MJ was quantified in fruit at zero, three and six days after harvest.The effects of MJ on ethylene biosynthesis were tested by incubating strawberry discs (2 cm diameter, 3 mm thickness) at white, halfripe and at fully ripe stage in petre dishes containing 20 ml mannitol (0.4 M) with 0, 10 and 50 mM MJ for 24 or 48 hours, followed by transfer of discs to MJ-free petridishes.Ethylene production, ACC synthase and ACC oxidase activities were measured at zero, one, two, and three days after treatment.Trans-methyl jasmonate was detected in strawberry fruit using GC-MS at white, half ripe and at fully ripe stages.The concentration of endogenous MJ was significantly higher in the white fruit (31.7-162.2 ng g
-1) and decreased ethylene production was recorded in strawberries when MJ was applied to white, half ripe and at fully ripe fruit.The application of MJ (50
mM) resulted in significantly highest ethylene production and increased activities of ACC synthase and ACC oxidase as compared to all other treatments.The effects of exogenously applied MJ on ethylene production, ACC synthase and ACC oxidase activities was dependent on concentration of MJ and fruit developmental stage.