บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Expression analysis of ripening-related genes in strawberry fruits with altered auxin metabolism

Trainotti L., Costantini E. and Mezzetti B.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004. 143 p.

2004

บทคัดย่อ

Expression analysis of ripening-related genes in strawberry fruits with altered auxin metabolism.   Strawberry is a false fruits with a non-climacteric ripening habit and it is well known that auxin does control some aspects of fruit ripening.By altering the endogenous content of auxin in the ovules, the DefH9-iaaM gene triggers the formation of parthenocarpic fruits in Solanaceae.Furthermore, DefH9-iaaM strawberry and raspberry plants grown under standard cultivation conditions show a drastic increase in fruit productivity. In all three Rosaceae species tested, Fragariavesca, Fragaria x ananussa and Rubus idaeus, the weight and size of DefH9-iaaM transgenic fruits was increase.Moreover, DefH9-iaaM plants have an increased number of flowers/fruits per inflorescence and an increased number of inflorescences per plant.

We have started to investigate if the increase in auxin contents, already observed in young developing fruits of DefH9iaaM clones of F.vesca (cv. Alpina) and F. xananassa (a breeding selection AN93.231.53), can in some way alter the ripening syndrome.Since the softening is a very important quality trait of the strawberry fruits, we have studied possible effect of the transgene o­n this process by analyzing the expression of genes encoding cell wall degrading enzymes.

Northern analysed were carried out o­n total RNA extracted from fruits at different stages of development and ripening.Of the different genes tested, FaEGl shows an earlier expression in fruits of the DefH9iaaM clone of F. x ananassa, compared to the control o­nes.This difference was not detected between the Control and GM clones of F.vesca, probably because of the shorter period of fruit growth and size in comparison with F. x ananassa.

Since it was previously observed that the FaEGl gene is negatively regulated by auxin, a discussion will be presented o­n the possible cause of such an anticipation of the softening in the transgenic strawberries.