บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Functional genomics and biotechnology of broccoli yellowing associated genes.

Shaw J.F.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004. page 34

2004

บทคัดย่อ

Functional genomics and biotechnology of broccoli yellowing associated genes. Broccoli (Brassica oleracea) florets senescenceand turn yellow rapidly after harvest.Subtractive hybridization technique was used to clone over 300 cDNAs with enhanced expression during floret senescence.These include genes involved in cell wall degradation (pectinesterase, polygalactruonase, pectin methylesterase), chlorophyll degradation (chlorophyllases), carbohydrate metabolism (fumarase, aconitase, sucrose synthase, fructose-biphosphate aldolase, pyruvate dehydrogenase), protein and amino acid metabolism (asparagines synthase, glutamine synthase, glutamate dehydrogenase, cysteine protease), nucleic acid metabolism (hypoxanthin-guanine phosphoribosyltransferase, bifunctional nuclease), lipid metabolism (beta-ketoacylthiolase, oleosin-like protein, digalactosyldiacyglycerol synthase, fatty acid binding protein), signal transduction (ethylene receptors, protein kinases, jasmonic acid regulatory protein, A. thaliana mRNA protein kinase), enviromental and ocidative stress (metallothionein (MT1 and MT2), cytochrome P450, antifungal protein, catalase, 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase), drought-induced protein, 1,2-dioxygense, GA-stimulate transcript (GAST1), gama-glutamylcysteine synthase), and many other unknown functional genes.Some of these senescence associated genes have been cloned, expressed and characterized, including broccoli ACC oxidase, cystein protease, caffeoyl CoA 3-o-methyltransferase, chlorophyllase, ethylene receptors (ETR1 and ERS), CTR1, metallothionein, bifuctional nuclease and antifungal protein and chlorophyllase will be discussed.

A broccoli floret senescence-associated cDNA microarray was used to analyze the expression profile of these genes during senescence.A mutated broccoli boers-1 gene has been successfully transferred into broccoli and the floret yellowing was delayed for 3 days.Similarly, the mutated boers gene was transferred into petunia and the excised flowers remained fresh for 18 days, or five times longer than those of the control.