บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Mode of action of heat treatment applied to sweet basil (Ocimum basillicm) for reduction of chilling injury.

Faure Mlynski M., Aharoni N., Mayak S., Lers A.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.50

2004

บทคัดย่อ

Mode of action of heat treatment applied to sweet basil (Ocimum basillicm) for reduction of chilling injury.    Sweet basil (Ocimum basilicum) is a tropical crop sensitive to low temperatures.When basil is stored below 12 °C chilling injuries (CI) are developed leading to serious limitation for postharvest storage.The CI is manifested in the leaves as black dots or necrotic spots, browning of upper young leaves and wilting.Damage severity depends o­n storage temperature and length of exposure.Postharvest heat treatment was found to improve basil tolerance to low temperature.

Our research is aimed at identifying biochemical and molecular mechanisms involved in mediating the heat pretreatment for reducing basil chilling sensitivity.Possible involvement of the antioxidative system is suggested by the increase in observed general reductive potential in the leaves, as well as induction of super oxide dismutase (SOD) activity following heat treatment and cold storage.To identify candidate genes involved in the induced chilling resistance we have performed a PCR-select analysis.Several cDNAs were identified which represent genes induced in heat treated leaves compared to control.Two of these cDNAs, Ribulose 1-5 bisphosphate carboxylase oxygenase and Calcineurin B-like calcium sensor proteins (CBL) kinase, were previously suggested to be involved in plant response to abiotic stresses.

The Rubisco activase was suggested to be involved in plants tolerance to heat acting as a chaperone, while CBL kinase belongs to a protein family that function as signal sensors which has an important role in plant response to different stresses.Expression analysis of these genes in basil supports their involvement in induction of chilling resistance by the heat treatment.