บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Functional markers as genetic approach to study ethylene production and fruit softening in apple (Malus x domestica Borkh.).

Costa F., Stella S., Van de Weg W.E., Sansavini.

Costa F., Stella S., Van de Weg W.E., Sansavini., p.64

2004

บทคัดย่อ

Functional markers as genetic approach to study ethylene production and fruit softening in apple (Malus x domestica Borkh.). To date different types of molecular markers have been used in genetic studies o­n agronomic traits.These markers are usually interspersed over the entire genome and their trait association is determinated by linkage analysis.In contrast, functional markers are located within a target gene.Their allelic variation is directly associated with sequence differences of this gene.If these sequence differences effect phenotype, the these markers represent different functional alleles of the gene that controls the expression of a (complex) trait.In order to investigate particular aspects of fruit quality, three functional markers relative to ACO and ACS family gene members, which are involved in the ethylene pathway, and to an expansion gene member, which plays a key role in fruit softening, have been positioned o­n molecular marker linkage maps (Fuji x Mondial Gala and Prima x Fiesta) and evaluated for their involvement o­n Fuji x Braeburn progeny phenotype.

Md-ACO1 mapped o­n linkage group 10 of cvs.Fiesta and Mondial Gala.On Fiesta map, this marker was placed o­n the border of the 5% confidence region of a known QTL for firmness (King et al. 2000).

Md-ACS1 was mapped o­n LG15 of Prima and Fiesta, but was not associated with any known QTL for apple firmness.Each Fuji x Braeburn offspring that was homozygous for allele Md-ACS1-2 showed low levels of ethylene synthesis and extremely good retention of fruit firmness and shelflife.

Md-EXPDCA1 o­n LO1 of Prima, was mapped at approximately 9 cM of the Vf gene and was strongly correlated with a known QTL for crispness and juiciness (King et al.2000.).Comparative mapping among apple and pear maps showed an interspecific synteny for Md-EXPDCA1 marker which includes a microsatellite motif, tested o­n 44 apple and two pear genotypes.