Ethylene in the protection of citrus fruits to chilling induced damage.
Zacarias L., Lafuente M.T., Gosalbes M.J., Sanchez-Ballesta M.T., Marcos J.F., Grabnell A.
5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.76
2004
บทคัดย่อ
To understand the mode of action of ethylene in the response of Fortune mandarin to the chilling-induced damage, the effect of a pre-treatment with 1-MCP and of a continuous application of ethylene during fruit storage at 1 °C on chilling injury was studied.Inhibition of ethylene action accelerated the development of chilling injury whereas in an ethylene-atmosphere the cold induced damage was delayed.The effect of these treatments on the expression of ; 1) cold-induced genes, 2) genes that may be involved in the tolerance to cold induced by a heat conditioning treatment (3 days at 37 °C), and 3) genes of the ethylene biosynthetic pathway, all of them previously isolated in our laboratory, were further examined.Some of the cold-induced genes showed an ethylene-dependent expression but an ethylene-independent expression was also inferred.
Interestingly, some genes whose expression was enhanced by the heat treatment or by the combination of heat and cold were not clearly associated to the ethylene-induced cold tolerance.Genes involved in ethylene biosynthesis, ACC synthase (ACS) and ACC oxidase (ACO) were differentially regulated by ethylene during cold stress.The potential involvement of the isolated cold and heat-regulated genes in the ethylene-induced protection to chilling injury will be discussed.