บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

The regulation of ethylene production during fruit ripening in japanese pear.

Itai A., Tanabe K.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.76

2004

บทคัดย่อ

The regulation of ethylene production during fruit ripening in japanese pear.   Ethylene production by cultivated Japanese pear (Pyrus pyrifolia Nakai) fruits varied from 0.1 nL/g. f.w./hr to 300 nL/g.f.w./hr during ripening.This data indicate that there are both climacteric and non-climacteric cultivars of Japanese pear.Climacteric-type fruits exhibit a rapid increase in ethylene production and have a low storage potential, while non-climacteric fruits show no detectable ethylene production and fruit quality is maintained for over a month in storage.Thus, fruit storage potential is closely related to the amount of ethylene produced.

To elucidate the reason for the large differences in ethylene production among cultivars, we have cloned three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (PPACS1, PPACS2 and PPACS3) and studied their expression during fruit ripening in Japanese pear.PPACS1 is specifically expressed in cultivars with high ethylene production, while PPACS2 is specifically expressed in cultivars exhibiting moderate ethylene production.Moreover, we have identified two RFLP markers tightly linked to the locus conferring the ethylene pro9duction of ripening fruit, using RFLPs were designated as marker A for PPACS1, linked to the high levels of ethylene and marker B for PPACS2, linked to moderate levels of ethylene.The absence of these two markers enabled the identification of low ethylene producers.

Using these markers, we have identified ethylene genotypes for 40 Japanese pear cultivars that are commercially important and used in breeding programs.This information is very useful for the marker assisted selection (MAS) of Japanese pear seedling with good storage potential.