Changes in glutamine synthetase activity and contents during storage of vegetable soybean.
Matsui T.
5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004
2004
บทคัดย่อ
Changes in glutamine synthetase activity and contents during storage of vegetable soybean.
Vegetable soybean [Glycine max (L.) Merr., cv. Iwatemidori] deteriorates rapidly after harvest and is associated an decrease in the amino acid contents of the seed.In other plant systems it is thought that ammonia build up is toxic and causes tissue deterioration.Gltamine synthetase (GS, EC 6.3.1.2) is the key enzyme responsible for assimilating ammonia.As the first step towards controlling the loss of amino acid and possibly improving the postharvest life, the respiration rate, some amino acid and ammonia levels and the activity of GS in harvested vegetable soybean were examined.Vegetable soybean had been held at 5 and 20 °C up to 10 days.The respiration rate of the seed declined rapidly and it was lower at 20 °C than at 5 °C.Although GS activity remained constant at both temperature, the amino acid contents decreased rapidly as the time proceeds.The amino acid up to 3 days storage showed higher content at 5 °C than that at 20 °C.GC activity was higher at 20 °C since GS activity was controlled at lower temperature storage.