บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Evaluation of chilling damage in tropical vegetables with three procedures

Chucheep K., Kanlayanarat S., Nakamura Y., Okamoto S. and Matsuo T.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004. 143 p.

2004

บทคัดย่อ

Evaluation of chilling damage in tropical vegetables with three procedures.    Most tropical plants, including vegetables and fruits, are sensitive to chilling, which cause chilling injury under lower temperatures in the range of 0 °C to 12 °C.A better procedure, which is more sensitive and not time-consuming has been required to evaluate the chilling damage.The aim of this investigation was to adapt the WST-1 assay into a simple new procedure for examining chilling damage in seedlings of tropical vegetables and to compare it with two traditional procedures: measurement of ion leakage and seedling growth.

WST-1, which produces a highly water-soluble formazan dye after reduced by metabolically active cells, was applied to measure cell viability of mungbean seedlings.The result showed high correlation between chilling damage and WST-1 reduction activity as quantified by spectrophotometer at 450 nm.The longer exposure of seedlings to chilling temperature the lower the amount of water-soluble formazan dye yield by WST-1 reduction was.The evaluation was also very close to the other results obtained by ion leakage and growth.

Our results indicated that this WST-1 assay becomes a useful tool to measure the cell viability or cell damage in tissues or organs of higher plants treated by chilling and freezing.