บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Separation of cell wall associated enzyme solution of sour cherry (Prunus cerasus L.)

Kovacs E.; Nemeth-Szerdahelyi E. and Horvath S.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004. p 97.

2004

บทคัดย่อ

Separation of cell wall associated enzyme solution of sour cherry (Prunus cerasus L.)  The softening of fruit during ripening has been associated with alterations in the cell structure of wall and middle lamella. The hydrolysis of the pectin catalyzed by different enzymes. b-galactosidase was selected for analysis. This enzyme appears to exist galactose throughout fruit growths and development and for the degradation of galactose containing polysaccharides during senescence.

Different sour cherry cultivars (…rdi bitermi Kantorjanosi, Pandy 279) were investigated at harvest. Enzyme solution was prepared by Kavacs et.al. (1997). SDS-PAGE was performed using a mini gel system (Mini-Protean II Dual Slab, Bio-Rad). The protein bands were evaluated by a Biotec Fisher video densitometer. For identification b-galactosidase from Saccharomyces fragilis (EC 3.2.1 23, Sigma) and molecular mass markers (Pharmacia) were used. Electrophoresis and Protein blotting was used for identification of b-galactosidase were used according to Kovacs and Nemeth-Szerdahelyi (2002). The total protein content was determined according to the method of Bradford (1976).

The main observations were: the activities of b-galactosidase were different among cultivars; the enzyme consisted of different protein bands (16 kDa, 22 kDa, 50 kDa) separating by SDS-PAGE. The isoelectric focusing pattern of the crude enzyme solution showed differences of cultivars (Pandy 279 has less protein bands, than the others).