บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Efficacy and molecular characterization of Metschnikowia pulcherrima strains against postharvest diseases of pome fruit

Spadaro D.; Garibaldi A. and Gullina M.L.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.104

2004

บทคัดย่อ

Efficacy and molecular characterization of Metschnikowia pulcherrima strains against postharvest diseases of pome fruit  During the last decade several yeast strains have been selected for their antagonistic properties o­n postharvest biological control of fruit. Recently some strains of the yeast Metschnikowia pulcherrima, which proved to be effective in containing Botrytis and Penicillium spp. rots in apple were selected and studied. The main mode of action involved in the biocontrol is competition for nutrients or space although a direct interaction can not be excluded. In this study twenty-six strains of M. pulcherrima coming from different sources were studied for their biocontrol activities: nine strains were isolated from the carposphere of pome fruit, two from the carposphere of stone fruit, eleven from different steps in the wine production chain, four from unusual or unknown origin.

The strains were tested for their efficacy in the control of Botrytis cinerea and Penicillium expansum, causal agents of grey and blue mould o­n apple. In general the strains were more effective against B. cinerea. It was shown that antagonistic properties for biological control in the carposphere can be possessed by microorganims isolated from the same source where they will be applied as antogonists but there features can be owned also by microorganisms of different origin.

Molecular tools can assist to monitor the genetic and environmental fate of these agents after releasing. Moreover suitable and reproducible strain authentication methods are necessary in commercial procedures such as filling patents and product licensing. The selected strains were compared for their genetic diversity, using AFLP and RAPD technique. Starting from AFLP markers, STS (sequence tag site) primers for PCR-amplification of genomicDNA are under development.