ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Antifungal effect of enantiopure imazalil isomers on green mold, Penicillium digitatum, in citrus fruit

Axelrod Y.; Sasson Y. and Cohen E.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.113

2004

บทคัดย่อ

   Where consumers and health issues are involved, lowering the amount of chemicals o­n fresh perishable fruit and vegetables is a priority. Our research promises significant reduction of fungicide concentration in water solutions and wax emulsions used for treating fruit in packinghouses, thus reducing fungicide residues o­n the treated fruit.

Penicillium digitatum is a post-harvest wound parasite, which inoculates and infects citrus fruit in the grove, in packinghouses and storage rooms. The fungicide Imazalil belongs to the Imidazol group, an Ergosterol Biosynthesis Inhibitor (EBI) that effectively controls most anatomical stages ofPenicillium digitatum. Imazalil is worldwide use for postharvest treatment in Citrus Banana as well as other fruits and vegetables.

The aims of the research were twofold: to separate the optical isomers of common Imazalil and distinguish between them, and to select the most effective isomer which, at lowest concentration, would effectively control the disease.

Imazalil enantiomers (R) and (S) were separated via diasteroselective crystallization from the common (RS) imazalil salt formed between racemic imazalil and camphor sulfonic acid. Preliminary in vitro examination revealed that the separated (R) isomer inhibited mycelial growth and sporulation of P. digitatum better than the original (RS) imazalil. While both the (S) isomer and the commercial (RS) imazalil inhibited the fungus at 5.0 ppm. the(R) isomer completely inhibited the fungus at 1.0 ppm. Further in vitro and in vivo examinations supported the above results.