บทคัดย่องานวิจัย

Multispectral laser induced fluorescence imaging techniques for nondestructive assessment of postharvest food quality and safety

Kim M.S.; Lefcourt A.M. and Chen Y.R.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.117

2004

บทคัดย่อ

Multispectral laser induced fluorescence imaging techniques for nondestructive assessment of postharvest food quality and safety  The use of spectral sensing has gained acceptance as a rapid means for non-destructive inspection of postharvest food produce. Current technologies generally use color or a single wavelength camera technology. The applicability and sensitivity of these techniques can be expanded through the use of multiple wavelengths. Reflectance in the visible (Vis)/near-infrared (NIR) regions is the prevalent spectral technique. Fluorescence, compared to reflectance, is regarded as a more sensitive technique due to its dynamic responses to subtle changes in biological entities. Our laboratory has been exploring fluorescence as a potential means to detect quality and safety aspects of postharvest food produce.

We have developed several fluorescence imaging platforms including hyperspectral imaging, and laser-induced fluorescence (LIF) and steady-state fluorescence imaging systems with multispectral capabilities. Because of relatively low fluorescence quantum yield from biological samples, a system with a powerful and fast pulse light source such as a laser coupled with a gated defection device is used to harvest fluorescence in the presence of ambient light.

The versatilities of fluorescence techniques are demonstrate with recently developed multispectral LIF imaging systems, including nanosecond-scale time resolved fluorescence imaging, for the assessment of food quality and detection of fecal contamination of fruits and vegetable.