บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

A customized citrus microarray and its use in postharvest

Pons C.; Roya C.; Forment J.; Gadea J.; Lluch Y.; Kanellis A.K.; Zacarias L.; Lafuente M.T. and Granell A.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.128

2004

บทคัดย่อ

A customized citrus microarray and its use in postharvest The molecular mechanisms underlying the way fruit responds to the environment are not well understood, and this is especially true for those responses affecting fruit quality during storage. The development of high throughput molecular technologies such as transcriptomics can be very useful in defining the processes which affect quality during postharvest. We are applying microarraytechnology to Citrus varieties such as Fortune mandarin that develops peel alterations during extended cold storage with the idea the results can contribute to design better managing and storage procedures.

Although there are o­ngoing genomic programs in Citrus aim at cloning and identifying Citrus ESTs, these not yet available. Furthermore large scale ESTs projects use random selection of ESTs from a range of different libraries which obviously cannot include plant material under all possible conditions; thus the coverage of the array can not be complete what may be particularly relevant when the number of ESTs is not large as it is the case so for Citrus. In order to cover this gap we have constructed a cDNA microarray from Fortunemandarin fruits. The array includes random cDNAs, high/low oxygen response genes, but is enriched with cDNAs from a subtracted library that contains cold induced genes. Bioinformatics analyses of the results obtained with this array reveales a host of new genes associated to the response of Fortune fruit to low temperatures. Some of genes identified as cold responsive genes are tissue specific and are being classified by their pattern of expression and nature of the predicted gene product. The use of the array to study fruit response, in varieties differing o­n chilling response, in fruits exposed to different temperatures and time of storage, etc., will help us to get a more defined picture of the way storage conditions affect Citrus fruit quality.