Functional genomics and biotechnology of papaya maturation and ripening associated genes
Chen Y.T.; Chou T.L.; Yang C.Y.; Lai H.Y.; Jian W.T. and Shaw J.F.
5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.129
2004
บทคัดย่อ
Functional genomics and biotechnology of papaya maturation and ripening associated genes
Papaya, an important economic crop, its growth, development, and fruit ripening are all regulated by ethylene. From the orchard to market, the senescence and quality loss of papaya fruits are rapid. Subtractive hybridization method was used to clone 426 cDNAs (CpMRGs, papaya maturation and ripening associated genes) which are upregulated during papaya fruit ripening.These include genes involved in cell wall degradation, carbohydrate metabolism, protein and amino acid metabolism, nucleic acid metabolism, lipid metabolism, signal transduction, environmental and oxidation stress, light sensing, and many other unknown functional genes. The unknown functional cDNAs including 59 genes similar to Arabidopsis unknown proteins, 6 genes similar to
Zea mays proteins, and 272 genes without similarly to the genes deposited in data bank. Several papaya genes have been colned, expressed and characterized. These include 2 ACC oxidases, 3 ACC synthases, an antifungal protein, a chitinase and two tubby-like proteins. Antisense ACO gene has been successfully transferred into papaya and the fruit ripening of transgenic papaya was delayed for several days.
The 402 unique CpMRGs-based microarray was used to study the fruit-ripening associated genes. Forty two fruit specific and several unknown functional genes, and 4 genes, including heat shock protein 82, chitinase, 232 phospholipase, and HSC71.0 were up-regulated not only during fruit ripening but also during leaf and flower senescence.