ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Genomic approaches to postharvest biotic and abiotic stresses of citrus fruit

Gonzalez-Candelas L.; Sanchez-Torres P.; Alamar S.; Estables B.; Ballester A.R.; Sanchez-Ballesta M.T.; Lluch Y.; Gosalbes M.J.; Granell A.; Zacarias

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004

2004

บทคัดย่อ

Citrus fruit are subjected to both biotic and abiotic stresses during postharvest handling and storage that influence their marketability. The major biotic stress is caused by phytopathogenic fungi of the genus Penicillium, being P. digitatum the most important pathogen. Among the abiotic stresses, we are particularly interested in the heat-induced chilling tolerance of chilling-sensitive citrus cultivars.

To gain a deeper knowledge o­n the mechanisms underlying the defence response of citrus fruit to pathogen attack and o­n the heat-induced tolerance to chilling injury, we are undertaking two complementary approaches. In the framework of an Spanish “Citrus Functional Genomic Project, CFGP” we have generated o­ne cDNA library for each condition that will allow us to get a general picture of the genes being transcribed. A more directed approach is the utilization of the Suppression Subtractive Hybridization technique to generate subtracted cDNAs libraries enriched in those genes preferentially expressed under a particular condition. After a differential hybridization screening of the SSH libraries a group of clones were selected for further characterization.

We will present the results of both approaches, with special emphasis o­n the common responses found between the two different stresses, and within each stress o­n the comparison between non-selected ESTs versus subtracted libraries. All clones, together with those generated in the CFGP will form part of a microarray that will allow us a more detailed analysis of the citrus fruit responses to stress.