ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Differential gene expression analysis of two strawberry cultivars with a different metabolic response to storage in elevated concentration of CO2 using a tomato cDNA microarray

Ponce-Valadez M.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.130

2004

บทคัดย่อ

Some strawberry cultivars accumulate acetaldehyde, ethanol and ethyl acetate in response to high concentrations of CO₂ (20%) during storage, while in others the presence of these volatiles is not detectable. Our objective is to identify metabolic pathways other than fermentative that may be affected when cultivars of the two response types are exposed to high CO₂ concentrations. Microarray technology is a powerful tool that allows analysis of the expression of thousands of genes in a single experiment. However, generation of a species-specific microarray requires the construction of cDNA libraries, selection of expressed sequence tags (ESTs), sequencing., PCR and expensive equipment for spotting and processing.

With this in mind, we have utilized a publicly available tomato cDNA microarray with the knowledge that, although certain genes may be absent from the analysis, plants, and especially fruit, share many common biochemical pathways. The tomato microarray was hybridized with strawberry RNA extracted from two cultivars with different metabolic response to storage in CO₂. A set of 81 genes showed differential expression between the two cultivars when they were stored in an atmosphere containing 20% CO₂ for 48 hrs. Genes with homology to enzymes involved in cell wall metabolism, protein synthesis and stress were identified. An analysis of gene expression over time during storage will be presented from a subset of the genes identified from the microarray experiment.