ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Proteomic approach to analyse organoleptic quality in tomato fruit

Mihr C.; Faurobert M.; Pawlowski T.; Bouchet J.P. and Causse M.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.130

2004

บทคัดย่อ

The organoleptic quality of tomato fruits is a complex characteristic involving different components like flavour, aroma or texture. A recombinant inbred line (RIL) population was created from a cherry tomato line (Cervil) with a good overall taste and a common tomato line (Levovil) to study the genetic bases of these traits.

Molecular markers were used to map a total of 131 QTL for 38 traits which were distributed in different regions o­n chromosomes 2, 3, 4, 8, 9 and 12. For a particular region of chromosome 2 fine mapping was undertaken to separate four linked QTL o­ne for fruit weight, o­ne for locule number and two for sugar content. Near isogenic lines (NILs) for this special region were obtained. Three NILs as well as the two parental lines were compared during fruit development. Main variations were pointed out and compared with proteomes of other tomato tissues like roots, stems and leaves to detect fruit specific variations. To analyse the proteome, total protein extracts of the tomato pericarp were separated by two dimensional gel electrophoresis (IEF/SDS-PAGE). Proteins of interest were analysed by MALDI TOF or ESI-MS/MS and introduced into a protein database especially constructed for tomato fruit.

Proteomic analyses of parental lines and NILs were performed during different fruit developmental stages and candidate genes for the above mentioned characteristics were obtained. Some of these genes are well known to be linked to fruit development processes, the function of the others has to be further investigated.