Proteomics of genetic diversity in relation to tomato fruit Quality
Pawlowski T.; Faurobert M.; Mihr C. and Causse M.
5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.130
2004
บทคัดย่อ
Proteomics of genetic diversity in relation to tomato fruit Quality
Improvement of tomato fruit quality for fresh market is based upon accurate evaluation of the genetic variation easily accessible to tomato breeders. The quality of tomato fruit is a complex characteristic involving a set of components such as fruit size, flavor, aroma, texture. We here illustrate part of a research project aiming at comparing genetic diversity available by means of neutral molecular markers, transposable elements and expressed proteins within the
Lycopersicon genus.
Lycopersicon esculentum exhibits low molecular polymorphism but high phenotypic variability, especially at the fruit size level. We collected 20 accessions of esculentum varieties representing the overall existing fruit size variation (from few grams to 500g) and one accession of each wild species.
To analyse the proteome, total protein extracts of the tomato pericarp were separated by two dimensional gel electrophoresis (IEF/SDS-PAGE) at two different stages of development. Image analysis was performed with Melanie IV and main qualitative and quantitative variations were detected. Research of the co-regulated proteins was done by clustering analysis in relation to classes of fruit. Varying proteins were analysed by MALDI TOF or ESI-MS/MS and introduced into our tomato fruit protein database after comparison with proteins expressed in other tissue.