บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Microarray and real time PCR analysis of fruit transcriptome in strawberry elite genotypes and correlation with PTR-MS spectra of votalile compounds.

Carbone F.; Mourgues F.; Biasioli F.; Gasperi F.; Maerk T.D.; Rosati C. and Perrotta G.

5th International Postharvest Symposium . Volume of Abstract . Verona, Italy 6-11 June 2004, p.130

2004

บทคัดย่อ

Microarray and real time PCR analysis of fruit transcriptome in strawberry elite genotypes and correlation with PTR-MS spectra of votalile compounds.  We have been studying the transcriptome of the octoploid strawberry (Fragaria x ananassa) in a number of Italian elite varieties, to assess fruit quality and correlate gene expression data with those from biochemical analysis panel tests.

More than 3000 expressed sequence tags (ESTs) obtained from a fruit cDNA library were analyzed. The 50 largest contigs related to plant genes with assigned functions comprised 725 ESTs (23.7% of total), including genes not o­nly associated with ripening (cell wall metabolism, sugar and acid synthesis, pigment formation, vitamin synthesis and allergenic properties), but also with plant-pathogen interactions, abiotic stresses and housekeeping functions. Over 1800 selected ESTs were used to produce a cDNA microarray, together with ripening-related candidate genes and checks. Comparative profiling experiments revealed a limited number of genes with variations in expression levels among different genotypes. Nevertheless, such differentially expressed genes were related to important quality and post-harvest traits as fruit firmness and aroma profile, and data from microarray experiments were confirmed by results of Real Time PCR analyses.

Proton transfer reaction mass spectrometry (PTR-MS) analysis of head space volatile compounds of fruits of studied genotypes suggested a correlation between the presence in the fruit of some esterified metabolites and the expression of genes putatively involved in their synthesis. New results from o­ngoing molecular and biochemical analyses and correlation with data from panel tests will point out the genes and compounds most correlated to fruit quality and post-harvest traits, as well as overall consumers’ preferences.