Multivariate analysis of modifications in biosynthesis of volatile compounds after CA storage of ‘Fuji’ apples
I. Lara, J. Graell, M.L. López and G. Echeverría
Postharvest Biology and Technology Volume 39, Issue 1 , January 2006, Pages 19-28
2006
บทคัดย่อ
Emission of aroma volatile compounds and some related enzyme activities (LOX, PDC, ADH, and AAT) were assessed in ‘Fuji’ apples (Malus × domestica Borkh.) during shelf life at 20 °C following cold storage under air or under three different CA conditions (3 kPa O2:2 kPa CO2; 1 kPa O2:1 kPa CO2; or 1 kPa O2:2 kPa CO2). Data were used for principal component analysis (PCA) and partial least-square regression (PLSR) analysis of results. LOX activity was partly inhibited by hypoxic conditions, and thus could have contributed to differentiation between air- and CA-stored fruit. Accordingly, emission of straight-chain esters was also higher in air- than in CA-stored fruit. In contrast, PDC activity was responsible for part of the differences between low (3 kPa) and ultra-low (1 kPa) O2 storage conditions, probably by providing substrates for AAT action. AAT activity afforded no satisfactory differentiation between samples, and therefore it is suggested that substrate availability is a more decisive factor than enzyme activity for volatile production after storage. The PCA and PLSR models developed in this work were not useful for discrimination between the two studied ultra-low O2 conditions.