The action site of carbon dioxide in relation to inhibition of ethylene production in tomato fruit
Hans P.J. de Wild, Peter A. Balk, Elsa C.A. Fernandes and Herman W. Peppelenbos
Postharvest Biology and Technology Volume 36, Issue 3 , June 2005, Pages 273-280
2005
บทคัดย่อ
The action site of carbon dioxide in relation to inhibition of ethylene production in tomato fruitHigh CO2 can inhibit ethylene production of various fruit. A high level of CO2 (20 kPa) was applied to tomato fruit (Lycopersicon esculentum Mill. cv Aromata) at 18 °C for 5 days. To investigate the primary action site of CO2, we used tomato fruit at a ripening stage where feedback regulation of ethylene production was of limited importance. Feedback reactions were further prevented by a treatment with 1-methylcyclopropene (1-MCP) before exposure to high CO2. Tomatoes with and without 1-MCP pre-treatment were exposed to 0 or 20 kPa CO2. Ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) content and ACC oxidase mRNA abundance were measured after 1, 2 and 5 days exposure to 0 or 20 kPa CO2. High CO2-affected LE-ACO1, LE-ACO3 and LE-ACO4 transcripts differently. Several observations show that high CO2 did not affect the ethylene receptor: (1) CO2 had a much earlier and much stronger inhibitory effect on ethylene production than 1-MCP; (2) CO2 prevented while 1-MCP stimulated ACC accumulation; (3) CO2 prevented the 1-MCP induced decrease of LE-ACO1 abundance, and inhibited the 1-MCP induced decrease of LE-ACO3 abundance. Inhibition of ethylene production together