Isolation of Antagonistic Bacteria to Post-Harvest Phytopathogen Fungus in Papaya (Carica papaya L.) Var Maradol from Nayarit
C.H. Rodriguez-Cervantes, M.A. Vezquez-Arce, J.A. Ragazzn-Sanchez and M. Calderon-Santoyo
Book of Abstract. Information & technology for sustainable fruit & vegetable production, The 7th Fruit, Nut and Vegetable Production Engineering Symposium, 12-16 September 2005, Monpellier, France. Page 41
2005
บทคัดย่อ
An isolation of both pathogenic fungi and native bacteria from papaya was realized. During these essays, three out of the fire fungi founded in situ were considered as primary infection agents, these being later identified as Aspergillus sp. (H1), Penicillium sp. (H3) and Colletotrichum sp. (H5) after pathogenicity tests fulfilled Koch postulates. From the five bacterial genres isolated, two of these were considered as enteric bacteria after proper biochemical test were done, leaving Bacillus sp. (B1), Psedomonas sp. (B3) and Pseudomonas sp. (B5) as potential biocontrol agents against fungi. In vitro test showed that all of the bacterial genres used as biocontrol managed to inhibit all pathogens, but showing different inhibitory mechanisms. During In vivo tests, Pseudomonas sp. (B3) fail to control any of the three primary pathogens. However Bacillus sp. demonstrated control against Hl and H3 but not against H5. on the other hand, Pseudomonas sp. (B5) only controlled H5. Thus, in order to obtain full control of all the pathogens, a mix of B1+B5 was used with a complete inhibition of all pathogens during In vivo tests as a result The synergic effect reached with this mix ensures no resistance development from the pathogen, which is an advantage against the use of chemical control.